Category: Orchid Doctor

FLASKING FORMULAS: Knudson-c Medium

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 10 years ago Monopotassium phosphate 0.25gm., calcium nitrate 1.00 gm., ammonium sulphate 0.50 gm., magnesium sulphate 0.25 gm., ferrous sulphate 0.025gm., manganese sulphate 0.0075 gm., agar 17.5 gm., sucrose ... Read More

FLASKING FORMULAS: With Fungicides and Bactericides Included

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 10 years ago For the results of tests on the possible toxic effects of pesticides included in the flasking media, refer to W9-179 Read More

FLASKING: Air Supply to Seedlings

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 10 years ago For cymbidium seedlings in flask, air exchange must always be provided. OA84-126 Read More

FLASKING: Banana Added to Prepared Agar

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 10 years ago Ripe or green banana is homogenized in a kitchen blender; add 5% of it to the medium. A74-792 Read More

FLASKING: Best Procedure to Avoid Reflasking

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 10 years ago Sow the seed thinly in the original flask. A75-35 Read More

FLASKING: Black Substance in the Culture Medium

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 10 years ago Phalaenopsis seedlings are notorious for exuding a black material from the roots; it is not a contaminant but in high concentrations impedes growth. F82-175 Read More

FLASKING: Culture Medium for Terrestrial Cypripediums

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 10 years ago Excellent results reported with: Hyponex 3 grams; Difco-Bacto peptone or tryptone 2 grams, sucrose 35 grams, agar 15 grams, water 1000 ml. and pH adjusted to 5.0 A63-354+; A63-927 Read More

FLASKING: Delayed Contamination

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 10 years ago It is possible when a pathogen was introduced to the sterile flask and remained lodged near the opening; it would take severa1 weeks to reach the agar medium. F82-175 Read More

FLASKING: Detailed Working Instructions with Illustrations and Much Detail

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 10 years ago Refer to A60-597+; A64-759+; A70-599+; A75-109+; OD68-204+ Read More

ACID OR ALKALINE: Changing the Values

Author is Robert M. [Bert] Hamilton (Compiler), posted almost 10 years ago Various means can be used: tufa, a limestone form, can be added to paphiopedilums from limestone cliffs; bark composts acidify with age; peatmoss, pine needles, oak leaves in the mix produce acidit... Read More