GREEN POD CULTURE: Method

Culture Orchid Doctor

by Robert M. [Bert] Hamilton (Compiler)

Originally published in The Orchid Doctor in 1980 and 1988

Posted by Sys Admin almost 8 years ago.

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Surface sterilize the pod with 0.5% sodium hypochlorite for 20 minutes; cut open with a sterile knife; scrape out seed with sterile loop into Knudson-C agar or other nutrient solution; place in incubator for two months; transfer to light until differentiated, then transfer onto fresh agar, then treat as germinated dry seed. A72-1096
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Green Pod Culture Comments
GREEN POD CULTURE: Harvesting Time in Days Aerides 90, Brassavola 90-100, Broughtonia 30-45, Cattleya bifoliates 90; Cattleya unifoliates 120-135; Dend. nobile hybrids 90-100; others 60-75; Doritaenopsis hybrids 90-100; Epidendrums 150-165; Oncidiums 60-75; Phalaenopsis 90-100; Vandaa. 90-120. Fitch,All about orchids (1981)-203 0
GREEN POD CULTURE: Harvesting Times The time saved by comparison with seed culture is six to eight months; the optimum harvesting times for a long list of hybrids and species is given; refer to A76-305+; OD68-207 0
GREEN POD CULTURE: Of Phalaenopsis Technique; medium; culturing done 75 days after pollination of doritis and 85 for phalaenopsis; A62-819+; for embryo culture refer to A60-518 0
GREEN POD CULTURE: Time for Pod to Remain on Plant 100 days after pollination is sufficient for many genera. A65-909; A75-803 0
GREEN POD CULTURE: Time to Remove Wait till the flower end starts to turn yellow; aspasias, brassias and odontoglossums are long in ripening, up to ten months; many pods are lost because pods are removed too soon. OD77-101 0

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